Single-Cell Virology: On-Chip, Quantitative Characterization of the Dynamics of Virus Spread from One Single Cell to Another

Single-Cell Virology: On-Chip, Quantitative Characterization of the Dynamics of Virus Spread from One Single Cell to Another

Blog Article

Virus spread at the single-cell level is largely uncharacterized.We have designed and constructed a microfluidic device in which each nanowell contains a single, infected cell (donor) and a single, uninfected cell (recipient).Using a GFP-expressing poliovirus as our model, we observed eau micellaire both lytic and non-lytic spread.

Donor cells supporting lytic spread established infection earlier than those supporting non-lytic spread.However, non-lytic spread established infections in recipient cells substantially faster than lytic spread and yielded higher rates of genome replication.While lytic spread was sensitive to the presence of capsid entry/uncoating inhibitors, non-lytic spread was not.

Consistent with emerging models for non-lytic spread of enteroviruses using autophagy, reduction in LC3 levels in cells impaired M Shorts non-lytic spread and elevated the fraction of virus in donor cells spreading lytically.The ability to distinguish lytic and non-lytic spread unambiguously will enable discovery of viral and host factors and host pathways used for non-lytic spread of enteroviruses and other viruses as well.